Amber Valletta was born in Phoenix, Arizona and raised in Tulsa, Oklahoma. She got her start in the fashion industry when her mother enrolled her in modeling school at the age of fifteen. She appeared on the cover of magazines and in advertisements for Louis Vuitton, Calvin Klein and Versace and hosted MTV's House of Style with fellow model and friend Shalom Harlow.
She was presented on the November 1999 Millennium cover of American Vogue as one of the "Modern Muses”. Valletta's first role was in the comedy film Drop Back Ten (2000); Later that year, she played a supporting role in the thriller film “What Lies Beneath”. The film was directed by Robert Zemeckis and starring Harrison Ford and Michelle Pfeiffer. She appeared alongside Nicolas Cage in The Family Man. In 2003, she played as Celine in Danny DeVito's Duplex, starring Ben Stiller and Drew Barrymore, she also played as Meline in Raising Helen (2004), opposite Kate Hudson, Hayden Panettiere, Abigail Breslin, and Helen Mirren.
Valletta's major role was as Allegra Cole in Andy Tennant's Hitch (2005), for which she gained more attention. The film was released on February 11, 2005 and was a box office and critical success. She also had a role in Transporter 2, playing the mother of the kidnapped kid. She appeared in the comedy The Last Time, costarring Michael Keaton and Brendan Fraser. In 2007, she starred in the horror film Dead Silence as Ella Ashen. Later in 2007, she portrayed the character of Claire in Premonition, starring Sandra Bullock.
Valletta appeared as Angie in Gamer, opposite Gerard Butler. In 2011, she appeared as Lydia Davis on the ABC's television drama series Revenge.